Elastic properties of grafted microtubules

نویسندگان

  • Francesco Pampaloni
  • Gianluca Lattanzi
  • Alexandr Jonáš
  • Thomas Surrey
  • Erwin Frey
  • Ernst-Ludwig Florin
چکیده

We use single-particle tracking to study the elastic properties of single microtubules grafted to a substrate. Thermal fluctuations of the free microtubule’s end are recorded, in order to measure position distribution functions from which we calculate the persistence length of microtubules with contour lengths between 2.6 and 48 μm. We find the persistence length to vary by more than a factor of 20 over the total range of contour lengths. Our results support the hypothesis that shearing between protofilaments contributes significantly to the mechanics of microtubules. PACS numbers: 87.15.Ya, 87.15.La, 87.16.Ka, 36.20.Ey ∗ Corresponding author. FP and GL have equally contributed to this work.

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تاریخ انتشار 2008